Protein Sequence Analysis Toolkit

Enter Protein/Peptide Sequence

Sequence Features

Length: 0

Molecular Weight: 0 Da

Isoelectric Point: -

Extinction Coefficient: -

Amino Acid Composition:

Sequence Visualization

Amino Acid Properties

Hydrophobic Hydrophilic Acidic Basic Neutral Cysteine Proline

Secondary Structure

Helix Sheet Coil

Isoelectric Point

Calculated pI: 7.2

3 7 11

The isoelectric point (pI) is the pH at which the protein has no net charge.

Molecular Weight

Calculated MW: 12,345 Da

Molecular weight calculated from the sum of amino acid residues.

Extinction Coefficient

ε₂₈₀ (M^-1cm^-1): 44,720

Calculated from the number of Trp, Tyr, and Cys residues.

Hydrophobicity Analysis

Hydrophobicity profile calculated using the Kyte-Doolittle scale.

Secondary Structure Prediction

Predicted using a protein language model (ESM-2).

Disorder Prediction

Predicted intrinsic disorder using IUPred2A.

Solvent Accessibility

Relative solvent accessible surface area (RSA) prediction.

Transmembrane Regions

Predicted using TMHMM.

Signal Peptide Prediction

Prediction: Present (1-24)

Predicted using SignalP-6.0.

Motif Analysis

N-glycosylation (N-X-S/T) 3 sites

Phosphorylation (S/T/Y) 8 sites

SH3 binding (P-X-X-P) 2 sites

Common functional motifs identified in the sequence.

Cytotoxicity Prediction

Prediction: Low risk

Predicted using a machine learning model trained on cytotoxic peptides.

Saturation Mutagenesis Analysis

Select Motif for Mutagenesis

Mutation Impact Summary

Position	Mutation	Stability ΔΔG	Function Impact
N45	N45A	+1.2	Disrupts glycosylation
N45	N45D	+0.8	Disrupts glycosylation
N45	N45Q	-0.2	Maintains glycosylation

Sequence Optimization

Optimization Goals

Improve stability

Improve expression

Improve solubility

Maintain function

Protein Language Model Features

Select Model

Feature Extraction

Per-residue embeddings

Contact map

Evolutionary conservation